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Primary antibodies used in this study.

Journal: Autophagy

Article Title: NUPR1 maintains autolysosomal efflux by activating SNAP25 transcription in cancer cells

doi: 10.1080/15548627.2017.1338556

Figure Lengend Snippet: Primary antibodies used in this study.

Article Snippet: DNA sequences for shRNA are listed in Table S2 . table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Primary Antibody Dilution Ratio Supplier ATG5 (rabbit polyclonal) 1:1,000 Cell Signaling Technology, 2630 ACTB (mouse monoclonal) 1:3,000 Sigma-Aldrich, A-3853 BECN1(rabbit polyclonal) 1:2,000 Cell Signaling Technology, 3738 CASP3 (rabbit polyclonal) 1:1000 Cell Signaling Technology, 9665 CDKN1A/p21Waf1 (rabbit monoclonal) 1:2,000 Cell Signaling Technology, 9932 CDKN1B/p27Kip1(rabbit monoclonal) 1:2,000 Cell Signaling Technology, 9932 CDKN2A/p16INK4a (rabbit monoclonal) 1:2,000 Abcam, ab108349 CTSB (mouse monoclonal) 1:1,000 Santa Cruz Biotechnology, sc-365558 CTSD (mouse monoclonal) 1:1,000 Santa Cruz Biotechnology, sc-377299 Cleaved CASP3 (rabbit polyclonal) 1:1000 Cell Signaling Technology, 9664 Cleaved CASP7 (rabbit polyclonal) 1:1000 Cell Signaling Technology, 8438 Cleaved CASP9 (rabbit polyclonal) 1:1000 Cell Signaling Technology, 7237 FLAG (mouse monoclonal) 1:4,000 Sigma-Aldrich, F3165 GAPDH (rabbit polyclonal) 1:3,000 Trevigen, 2275 HA (rabbit monoclonal) 1:2.000 Cell Signaling Technology, 3724S LC3B (rabbit polyclonal) 1:2,000 Sigma-Aldrich, L7543 MTOR (rabbit monoclonal) 1:2,000 Cell Signaling Technology, 2983 NUPR1 (mouse monoclonal) 1:1,000 Abcam, ab87454 p-MTOR(S2448) (rabbit monoclonal) 1:2,000 Cell Signaling Technology, 5536 PCNA (rabbit polyclonal) 1:1,000 Abcam, ab18197 SNAP25 (rabbit polyclonal) 1:1,000 Abcam, ab109105 SQSTM1/p62 (rabbit polyclonal) 1:1,000 Cell Signaling Technology, 7695 VAMP8 (mouse monoclonal) 1:1,000 Santa Cruz Biotechnology, sc-166820 Open in a separate window Primary antibodies used in this study.

Techniques: